Comprehensive research on the Phytochemical Characterization of Various Parts of Elaeagnus umbellata Using Advanced Spectroscopic and Chromatographic Techniques and Evaluation of Their Bioactive Potential

Abstract

The therapeutic value of Elaeagnus umbellata stems from its diverse biological activities and abundant phytochemical content. The overarching goal of this study was to assess the bioactive potential of Elaeagnus umbellata by conducting a thorough phytochemical analysis of the plant's leaves, fruits, and bark using state-of-the-art spectroscopic and chromatographic methods. Qualitative and quantitative phytochemical analyses were performed on plant materials after systematic extraction using appropriate solvents. To separate and quantify the main phytoconstituents, the extracts were further purified and studied using chromatographic techniques, such as thin-layer chromatography (TLC) and High-Performance Liquid Chromatography (HPLC). The isolated compounds were structurally characterized using state-of-the-art spectroscopic methods, such as ultraviolet-visible spectroscopy, Fourier-transform infrared spectroscopy, and mass spectrometry. Furthermore, in vitro tests were used to evaluate the bioactive potential of the compounds and extracts, namely, their antioxidant and antibacterial properties. The results of this study support the long-held reputation of Elaeagnus umbellata as a therapeutic herb by providing new information on the phytochemical profiles of the various parts of the plant. Based on these findings, Elaeagnus umbellata should be seriously considered for use in the pharmaceutical and nutraceutical industries as a source of bioactive chemicals.